Composite

Part:BBa_K3823014:Design

Designed by: Yiying Zhang   Group: iGEM21_Tongji_China   (2021-10-02)


T7-SQR-J23110-SDO-AprBA-SAT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 190
    Illegal XbaI site found at 47
    Illegal XbaI site found at 3581
    Illegal SpeI site found at 2054
    Illegal PstI site found at 1771
    Illegal PstI site found at 2983
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 190
    Illegal NheI site found at 151
    Illegal NheI site found at 1722
    Illegal NheI site found at 1745
    Illegal NheI site found at 2568
    Illegal NheI site found at 2591
    Illegal NheI site found at 3473
    Illegal NheI site found at 3496
    Illegal SpeI site found at 2054
    Illegal PstI site found at 1771
    Illegal PstI site found at 2983
    Illegal NotI site found at 1525
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 190
    Illegal BglII site found at 2254
    Illegal BamHI site found at 184
    Illegal BamHI site found at 3786
    Illegal BamHI site found at 5241
    Illegal XhoI site found at 1534
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 190
    Illegal XbaI site found at 47
    Illegal XbaI site found at 3581
    Illegal SpeI site found at 2054
    Illegal PstI site found at 1771
    Illegal PstI site found at 2983
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 190
    Illegal XbaI site found at 47
    Illegal XbaI site found at 3581
    Illegal SpeI site found at 2054
    Illegal PstI site found at 1771
    Illegal PstI site found at 2983
    Illegal NgoMIV site found at 255
    Illegal NgoMIV site found at 679
    Illegal NgoMIV site found at 1980
    Illegal NgoMIV site found at 4260
    Illegal NgoMIV site found at 4431
    Illegal NgoMIV site found at 5114
    Illegal AgeI site found at 1174
    Illegal AgeI site found at 2001
    Illegal AgeI site found at 2794
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References